Cryo-Transmission Electron Microscopy in Korean Society of Microscopy

Cohomology of Cryo-Electron Microscopy

The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this paper, we show that the basic premise of cryo-EM—patching together 2-dimensional projections to reconstruct a 3-dimensional object—is naturally one of Čech cohomology with SO(2)-coefficients. We deduce that every cryoEM reconstruc...

Mathematics for cryo - electron microscopy

Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method for biological macromolecules. Cryo-EM was selected by Nature Methods as Method of the Year 2015, large scale investments in cryo-EM facilities are being made all over the world, and the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubo...

Electron Microscopy of Fowl Pox Virus

Biological cryo‐electron microscopy in China

Cryo-electron microscopy (cryo-EM) plays an increasingly more important role in structural biology. With the construction of an arm of the Chinese National Protein Science Facility at Tsinghua University, biological cryo-EM has entered a phase of rapid development in China. This article briefly reviews the history of biological cryo-EM in China, describes its current status, comments on its imp...

Adaptive nonparametric detection in cryo-electron microscopy

Cryo-electron microscopy (cryo-EM) is an emerging experimental method to characterize the structure of large biomolecular assemblies. Single particle cryo-EM records 2D images (so-called micrographs) of projections of the three-dimensional particle, which need to be processed to obtain the threedimensional reconstruction. A crucial step in the reconstruction process is particle picking which in...